Relationship between Toll-like receptor 4(TLR4) on the peripheral blood monocytes and serum TNF-α in patients with chronic severe hepatitis B
- VernacularTitle:慢性重型乙型肝炎患者外周血单核细胞TOLL样受体4的变化及与TNF-α的关系
- Author:
Xiuqing WEI
;
Zhuofu WEN
;
Yunwei GUO
;
Fengping ZHENG
- Publication Type:Journal Article
- Keywords:
Hepatitis B,chronic;
Receptors,cell surface;
Tumor necrosis factor-alpha
- From:
Journal of Chinese Physician
2008;10(7):886-889
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change of TLR4 on peripheral blood monoeytes (PBMCs) and its role in the pathogenesis of chronic severe hepatitis B. Methods The expression of TLR4 on 10000 CDI4 + PBMCs was determined by flow eytometer in 30 healthy control,31 patients with chronic hepatitis B and 30 patients with chronic severe hepatitis B. The level of serum tumor necrosis factor α(TNF- α) was determined by ELISA. Results The values of TLR4 on PBMCs and serum TNF-αof the groups of healthy control, patients with chronic hepatitis B and patients with chronic severe hepatitis B were 2.3±1.1,3.7±2.3, (6.9±4.1 ) mean fluorescence intensity (MFI) and (53.8±38.1 ), ( 164.3±89.9) and (359.8±140.0) ng/L. The TLR4 value in patients with chronic severe hepatitis B was signifi- cant higher than those in healthy control and the patients with chronic hepatitis B ( P <0.05). However, there was no significant difference between the patients with chronic hepatitis B and healthy control ( P > O. 05 ). TNF-α increased gradually and significantly from the healthy control to the patients with chronic hepatitis B and patients with chronic severe hepatitis B. There was a significant positive correlation be- tween the value of TLR4 and the value of serum TNF-αin the patients with chronic severe hepatitis B( r=0.666, P <0.01). Conclusion There may be a role of TLR4 in the pathogenesis of chronic severe hepatitis B.
