Neuropathulogical study on epilepsy in organotypic slice model
- VernacularTitle:癫(癎)脑片模型的建立及其病理学研究
- Author:
Jinbo DENG
;
Shanting ZHAO
;
Guimin WANG
;
Yanli NIU
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Hippocampus;
Apoptosis;
Mossy fibers,hippocampal;
Pyramidalcells
- From:
Chinese Journal of Neurology
2008;41(8):547-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to make a epileptic model in organotypic slice culture and to further investigate the epileptic pathology. Methods Immunohistochemistry, Fluoro-Jade B staining and BrdU labeling were carried out in the hippocampal slices with domoic acid treatment after 7 days of culture.Results Domoic acid could induce a series of pathological changes, such as, dispersion of granule cells(0.105±0.063) mm vs (0.057±0.012) mm, t = 4.8, P<0.01), neurogenesis in granular layer and cell loss of pyramidal cell and Mossy cell as well. Conclusions In hippocampal organotypic slices, domoic acid induced such pathological changes as human temporal lobe epilepsy and epileptic animal model. It is an ideal epileptic model for pathological, pharmacological and electronic physiological studies with great applicable prospect.
