Radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM-1685 on A549 human lung adenocarcinoma cells
- VernacularTitle:LM-1685对人肺腺癌细胞A549的放射增敏作用
- Author:
Liming SHENG
;
Yongjie SHUI
;
Xian ZHONG
;
Li SHEN
;
Qichun WEI
- Publication Type:Journal Article
- Keywords:
Lung cancer;
Radiotherapy;
COX-2 inhibitor;
Radiosensitization
- From:
Chinese Journal of Radiological Medicine and Protection
2008;28(4):323-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM-1685 on A549 cells in vitro.Methods A549 human lung adenocarcinoma cell line was used in this study.Cell growth kinetics Was determined using MTT assay.Cell survival was analyzed by clonogenic assay.The change of cell cycle Was measured by flow cytometry.Results LM-1685 inhibited the growth of A549 cells,showing a dose-dependent and time-dependent manner.LM-1685(50/μmol/L),either with or without IL-1β,showed the radiosensitizing effects on A549 cells,and the sensitizing enhancement ratio(SER)was 1.12 and 1.06,respectively.LM-1685(50 μmol/L)abrogated radiation-induced G2/M arrest of the tested A549 cells.Conclusions Cyclooxygenase-2 selective inhibitor can enhance the radiosensitivity of A549 cell line.Abrogation of radiation-induced G2/M arrest could be part of the mechanism.
