Expression of COP9, JAK2, HSP and NADH in ovarian carcinoma tissues after taxol-chemotherapy and their significance
- VernacularTitle:紫杉醇化疗后卵巢上皮性癌组织中COP9、JAK2、HSP、NADH基因表达的变化及其意义
- Author:
Hongxia LI
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Paclitaxel;
Drug resistance,neoplasm;
Peptide hydrolases;
Multipretein complexes;
Protein-tyrosine kinase;
Heat-shock proteins;
Polymerase chain reaction
- From:
Chinese Journal of Obstetrics and Gynecology
2008;43(7):528-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of COP9,JAK2,HSP and NADH genes in ovarian carcinoma tissues after taxol-chemotherapy and their significance. Methods The up-regulated genes of JAK2, HSP, NADH and the down-regulated gune of COP9, which were revealed by micro-array from our previous study were examined by RT-PCR and real-time-PCR in 33 cases of ovarian cancer who previously received taxol-based chemotherapy (group 1 ), and 21 cases of ovarian cancer who never received chemotherapy before operation ( group 2 ) . Results The expression rate of COP9 gene in group 1 was detected markedly lower than that in group 2 (39% vs 95%, P < 0. 01 ) ; whereas the expression rates of JAK2, HSP and NADH in group 1 were significantly higher that those in group 2 (91%, 97% , 94% vs 29%, 48%, 43% ; all P < 0. 05). And the expression of COP9, HSP and NADH genes had no significant differences among histological grades. However, a significantly higher expression of JAK2 gene was seen in grade 3 than in grade 1 -2 (P <0. 01 ). No significant difference in the expression rates of the 4 genes was seen among various tumor types or chemotherapy courses ( P > 0. 05 ). Real-time PCR showed that the level of COP9 gene copies of group 1 was significantly lower than that of group 2 (568, 1866 respectively; P <0. 05 ). However, HSP, JAK2 and NADH genes had significantly higher copy numbers in group 1 than in group 2 (5766,7653,3200 in group 1 and 3341,3094,1522 in group 2, respectively; all P < 0. 05 ). In the subgroup that received 6 - 10 chemotherapy courses, the copy concentrations of JAK2, HSP, NADH genes were higher than those in the subgroup that received 2 - 4 chemotherapy courses ( all P < 0. 05 ). In addition,we found a higher copy concentrations of JAK2, HSP, NADH genes in grade 3 than in grade 1 - 2 ( all P <0. 05 ). Though no significant differences in gene copy concentrations of the 4 genes were seen among variable tumor types. In stage Ⅳ, the copy concentrations of HSP and NADH genes were higher than those in stage Ⅲ ( P < 0. 01, P < 0. 05 respectively ), but the copy concentrations of COP9, JAK2 genes had no significant differences( both P > 0. 05 ). There were positive correlations among JAK.2, HSP and NADH genes ( r =0. 56,0. 44,0. 57 respectively ,all P <0. 01 ). COP9 gene was found to have a negative correlation with JAK2 gene ( r = - 0. 48 ; P < 0. 01 ), but not with HSP and NADH genes ( r = - 0. 18, - 0. 06, respectively;both P > 0. 05 ). Conclusion The down-regulation of COP9 gene and up-regulation of JAK2, HSP, and NADH genes are related to the mechanism of drug-resistance in ovarian cancer.
