HBV genotypes and gene mutations in patients with liver failure after withdrawal of lamivudine
- VernacularTitle:拉米夫定停药后出现肝衰竭患者HBV基因型及基因变异
- Author:
Xiaohong ZHANG
;
Bingliang LIN
;
Shaoji YANG
;
Zhiliang GAO
- Publication Type:Journal Article
- Keywords:
Liver failure;
Lamivudine;
Relapse;
Genotype;
Mutation
- From:
Chinese Journal of Clinical Infectious Diseases
2008;1(2):80-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate HBV genotypes and gene mutations in chrenic hepatitis B (CHB) patients with liver failure after lamivudine withdrawal. Methods Twenty four patients with relapsing CHB after lamivudine withdrawal were divided into liver failure group ( n = 12 ) and chronic hepatitis group ( n = 12 ). HBV DNA from these patients was amplified by PCR. The PCR products were cloned into PGEM-T vector and HBV DNA sequences were analyzed. Results In liver failure group, there were 6 sequences detected, in which 3 were of genotype B and 3 were of genotype C. In chronic hepatitis group, there were 9 sequences detected, in which 2 were of genotype B and 7 were of genotype C. Compared with the wild type HBV sequences, there were multiples mutations in S, P, C, X regions. Gene mutations in high conservative sequences of BCP and P regions were detected in liver failure patients after lamivudine withdrawal. Conclusions In HBV patients with liver failure after lamivudine withdrawal, half of them were of genotype B and the others were of genotype C. Some mutations in high conservative sequences of BCP and P regions may be related to the liver failure in these patients.
