A population-based study of the inducible nitric oxide synthase gene polymorphism for stroke with coronary artery disease in a Chinese population
- VernacularTitle:诱导型一氧化氮合酶基因多态与脑卒中合并冠心病
- Author:
Danhua DU
;
Jiang WU
;
Peng GAO
;
Linsen HU
;
Jiexu ZHAO
- Publication Type:Journal Article
- Keywords:
Cerebrovascular accident;
Coronary disease;
Nitric oxide synthase type Ⅱ;
Polymorphism,single nucleotide
- From:
Chinese Journal of Neurology
2008;41(7):440-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic association between the inducible nitric oxide synthase (NOS) 2A gene and stroke with a history of coronary artery disease ( CAD). Methods 708 patients with stroke and 235 healthy controls were recruited in this study, and the stroke group was delaminated into 2 subgroups according to the history of CAD. SNP rs28944190, an A to C base change located in intron 22 of the gene, was used as a genetic marker. PCR-based restriction fragment length polymorphism analysis was applied to genotype rs28944190 (Hac Ⅲ site). Results The x2 test showed no association between patients with stroke and healthy controls. Of 708 patients, 94 had a history of CAD and the frequency of allele C of rs28944190 was significantly higher in patients with a history of CAD than those without (23.9% vs 16.6%, x2 =5.629, df= 1, P =0.018, OR = 1.580, 95% CI 1.083—2.306), especially in male patients (x2 = 8. 592, df= 1, P = 0. 003, OR = 1. 983, 95% CI 1. 255—3. 134). The frequency of genotype AA + AC of rs28944190 was significantly higher in patients with a history of CAD than those without such a history (47.9% vs 30. 8%, x2 = 10. 761, df= 1, P = 0. 001, OR = 2. 065, 95% CI 1.34—3.19), especially in male patients (x2 = 15. 762, df= 1, P =0. 000, OR =2. 985, 95% CI 1.74—5. 12). Conclusion The present study suggests that the NOS2A gene is unlikely to contribute to the etiology of stroke.
