The inhibitory effect of Matrine on the proliferation of peripheral lymphocytes of patients with rheumatoid arthritis
- VernacularTitle:苦参碱对类风湿关节炎外周血淋巴细胞增殖抑制细胞周期影响的研究
- Author:
Zhu CHEN
;
Xiaoying ZHANG
;
Xiaofeng LI
;
Hongyan WEN
;
Xiaoli WANG
;
Qianyu GUO
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Matrine;
Methotrexate;
Lymphocyte
- From:
Chinese Journal of Rheumatology
2008;12(8):546-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Matrine (Mat) on the proliferation and cell cycle of the peripheral blood lymphocytes of patients with rheumatoid arthritis.Furthermore,its mechanism of treatment for rheumatoid arthritis (RA) is explored by comparing with methotrexate (MTX).Methods MTr method was used to measure the light-absorption value of the cells treated with different doses of Mat and MTX at different time,and the inhibition rate(IR% ) was calculated.Flow cytometry was used to measure cell cycle of the cells treated with different doses of Mat and MTX for 48 h.Results ① Both MTX and Mat could inhibit the proliferation of PBL in patients with RA and the effect was dose-dependent.The IR%was increased (P＜0.05).IR% at 48 h was higher than IR% at 24 h (P＜0.01).The two medications had no significant difference (P＞0.05).② Both MTX and Mat could change the cell cycle and acted on G1/S point.They both increased the number of cells in G0/G1 phase and decreased cells in S and G2/M phase (P＜0.05).The two medications had no significant difference (P＞0.05).Conclusion Mat inhibits the proliferation and proliferation-dependent processes of PBL in patients with RA.Its effect is comparable to MTX.Mat may have the potential in the trea-tment of RA.
