The effect of diabetes on expression of the glucose transporter-1 in blood brain barrier endothelial cell of diabetic rats at different time points
- VernacularTitle:不同时期糖尿病对大鼠血脑屏障内皮细胞葡萄糖转运蛋白-1的影响
- Author:
Shiwei LIU
;
Ling YIN
;
Xia Lü
;
Yuwen SHANG
;
Meikui ZHANG
;
Kan DU
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,experimental;
Glucose transporter type 1;
Blood-brain barrier;
Endothelial cells
- From:
Chinese Journal of Neurology
2008;41(7):482-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of diabetes on the expression of the glucose transporter (GLUT-1) in blood brain barrier (BBB) endothelial cell at different time points. Methods Forty male SD rats were randomly divided into normal control and diabetic group. Diabetic model of SD rats was established by intravenous streptozotocin(50 mg/kg) injection. Ten rats in each group were sacrificed on the 2nd month and 10th month respectively. The rat' s prefrontal cortex was removed to observe the expression of GLUT-1 in BBB EC with immunohistochemical(IHC) staining. Results By IHC method, the number of GLUT-1 positive microvessels was significantly decreased in diabetic group in the 2nd month (4. 3 ± 0. 9) compared with that of control group (6.4 ± 1.7, t = 7. 586, P < 0. 01 ) ; the number of GLUT-1 positive microvessels was significantly increased in the diabetic group in the 10th month (8.4 ± 1.4) compared with that of control group (5. 8 ± 1.7, t = 15.922,P < 0. 01 ). Conclusions Short-term diabetes GLUT-1 expression in BBB EC is downregulated in early stage, while it is upregulated in advanced stage.
