Effects of emodin on expression of PMN Fas/FasL,Caspase-3 from peripheral blood in patients with SIRS
- VernacularTitle:大黄素对全身炎性反应综合征病人外周血中性粒细胞Fas/FasL、Caspase-3表达的影响
- Author:
Baozhi WANG
;
Dong SHANG
;
Manchao LI
- Publication Type:Journal Article
- Keywords:
Drug evaluatirm;
Systemic inflammatory response syndrome;
Apoptosis;
Polymorphonuclearneutrophils;
Emodin;
Fas/FasL;
Caspase-3
- From:
Chinese Journal of Hepatobiliary Surgery
2008;14(8):566-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of PMN Fas/FasL,Caspase-3 from peripheral blood in patients with SIRS and observe the effect of emodin on the PMN apoptosis and explore its mechanism.Methods The samples of peripheral blood were collected from 8 patients(all suffer from acute pancreatitis)with SIRS and 6 healthy volunteers.The circulating neutrophils were isolated and cultured.There were three groups of in our study:control group,SIRS group and emodin-treated group.The change of the PMN apoptosis and the expression Ieve]of Fas/FasL.Caspas-3 were observed.Results Compared with healthy volunteers.the percentage of PMN apoptosis significantly reduced in patients with S1RS(P＜0.05).After emodin was used,the percentage of PMN apoptosis in patients with SIRS increased(P＜0.05).Compared with healthy volunteers,the expression of Fas and Caspase-3 reduced in patients with SIRS(P＜0.05).Emodin could significantly induce the expression of Fas and Caspase-3(P＜0.05).After 24 hours of in vitro culture.the expression of FasL was not detected.Conclusion PMN from patients with SIRS shows profoundly delayed rates of apoptosis in vitro compared with PMN from healthy volunteers,and the decreased percentage of PMN apoptosis is associated with the reduced expression of Fas and Caspase-3.Emodin can significantly inhibit the delayed PMN apoptosis in patients with SIRS by inducing the expression of Fas and Caspase-3.
