Expression of mieroRNA-224 in HepG2 cells and analysis of its predicted target genes
- VernacularTitle:MicroRNA-224在肝癌HepG2细胞中的表达及其靶基因的预测分析
- Author:
Qiong LI
;
Ge WANG
;
Jinlu SHAN
;
Chuan CHEN
;
Zhimin ZHANG
;
Wen XU
;
Xizhong LUO
;
Dong WANG
- Publication Type:Journal Article
- Keywords:
MicroRNA-224;
Gene chip;
Target gene;
Liver cancer
- From:
Chinese Journal of Digestive Surgery
2008;7(4):297-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of microRNA-224 in HepG2 cells and analyze its target genes to reveal its role in the carcinogenesis of hepatoma. Methods The genes with differential expression in HepG2 cells and LO2 cells were obtained by gene expression microarray analysis. The up-regulated target genes of microRNA-224 were predicted by bioinformatics method, and their functions were analyzed. Results Compared with LO2 cells, microRNA-224 was highly expressed in HepG2 cells. A total of 264 target genes of microRNA-224 were predicted, including genes involved in cell cycle, signal transduction, cell differentiation, proliferation and apoptosis. Conclusions MicroRNA-224 is highly expressed in HepG2 cells. MicroRNA-224 plays an important role in the carcinogenesis of hepatoma via regulating the expression of its target genes directly or indirectly.
