The role of estrogen in the pathogenesis of lupus in NZB/w F1 mice by modulating the function of den dritic cells
- VernacularTitle:雌二醇调节树突状细胞功能参与狼疮鼠的发病机制
- Author:
Bo JIANG
;
Lingyun SUN
;
Hong WANG
- Publication Type:Journal Article
- Keywords:
17β-estradiol;
Dendritic cells;
NZB/w F1 mice;
Spleen
- From:
Chinese Journal of Rheumatology
2008;12(8):530-533
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of estrogen on dendritic cells (DCs) and its role in disease progression by comparing the effects of 17β-estradiol (E2) on spleen DCs in systemic lupus erythe matosus (SLE) murine modeI-(NZBxNZW) F1 (NZB/w F1 ) female mice before and after the disease onset.Methods Anti-CD11c antibody labeled magnetic bead was used to purify spleen DCs.Flow cytometry was utilized to detect the co-stimulatory molecules and intracellular cytokines of DCs.Mixed lymphocytes reaction (MLR) was used to measure the stimulatory activity of DCs to T iymphocytes.And semi-quantitative RT-PCR was employed to check the expression of estrogen receptor (ER).Results E2 could modulate the expression of surface molecule CD40,the production of cytokines IL-6,IL-10,IL-12 and TNFa,and the stimulatory a bility of spleen DCs in SLE model mice.Tamoxifen (TAM) could antagonize E2 effects and E2 could affect the estrogen receptor (ERα) level of DCs.These changes of DCs varied with age.Conclusion E2 may be in volved in the pathogenesis of SLE by modulating DCs by binding ERa.The effects of E2 vary in different stages of SLE progression.
