Expression of protein kinase B in 3T3-LI adipocytes in different insulin sensitivity status
- VernacularTitle:不同胰岛素敏感状态下3T3-L1脂肪细胞中蛋白激酶B的表达
- Author:
Minjie GUO
;
Lihong ZHOU
;
Enyi ZHANG
;
Junhua JIN
;
Huan GONG
;
Tiemei ZHANG
- Publication Type:Journal Article
- Keywords:
Insulin sensitivity;
Protein kinases
- From:
Chinese Journal of Geriatrics
2008;27(8):610-612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish cell models with different insulin sensitivity status and examine the expression of protein kinase B (PKB/Akt) in the same conditions. Methods 3T3-L1cells were cultured with different glucose concentrations (3.0, 5.5 and 50.0 mmol/L respectively).Insulin sensitivity, as presented by insulin-induced glucose transport rate in the cells, was tested by 3H-2-DG incorporation. PKB expression in cells was determined by RT-PCR. Results The insulin-induced glucose transport rate in 3T3-L1 cells was affected by different concentrations of glucose. The transport rate of 3H -2-DG in cells cultured in low glucose concentration was increased by 152% and 50% compared with high glucose concentration and control group, respectively. There was no difference in PKB mRNA expression among the three groups under the same conditions,whereas there was an obvious reduction of PKB expression in the three groups after the cells were treated with glucose and insulin. Conclusions 3T3-L1 cells cultured with different concentrations of glucose present the different insulin sensitivity status, but the difference of PKB mRNA expression has not been observed in the same condition.
