The immunoregulatory effects of thalidomide on T-lymphocytes of rheumatoid arthritis patients
- VernacularTitle:沙利度胺对类风湿关节炎T淋巴细胞的免疫调节作用
- Author:
Liwen YAN
;
Jing LI
;
Yingying QIU
;
Haiyan YOU
;
Zhijun JIAO
;
Zhong WU
;
Xiaoli SONG
- Publication Type:Journal Article
- Keywords:
Thalidomide;
Arthritis,rheumatoid;
T-lymphecytes;
Immunoregulatory
- From:
Chinese Journal of Rheumatology
2008;12(8):524-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunoregulatory effects of thalidomide on the peripheral blood T-lymphocytes of rheumatoid arthritis patients.Methods MTr was used to detect the effects of different thalidomide concentrations on the proliferation of T-cells.Flow eytometry was used to analyze T-cells early apoptosis and the T-cells subsets in different concentration of thalidomide.The mRNA expression of IL-6,IL- 10 and TNF-α was measured by RT-PCR method.Results The level of thalidomide at 500 μg/ml inhibited the proliferation of T-ceils and the CD3+CD28+ expression of T-cell subsets,but promoted the early apoptosis and the CD8+CD152+ expression of T-cell subsets.Thalidomine at any concentration could inhibit the mRNA expression of IL-6,TNF-α.However,the level of thalidomide that could promote the mRNA expression of IL- 10 was 100 μg/ml and 500 μg/ml.Conclusion Thalidomide can inhibit the proliferation of T lymphocytes and the expression of CD3+CD28+ on T-cell subsets.It can promote the early apoptosis and the CD8+CD152+ expression of T-cell subsets.Thalidomide inhibits the mRNA expression of IL-6 and TNF-α but promote the mRNA expression of IL-10.Thalidomide has immuno-regulatory effects on rheumatoid arthritis T-cells.
