Effect of recombinant human tumor necrosis factor receptor Ⅱ-Fc fusion protein on nuclear factor-κB protein expression and brain edema tranmalic brain injury in rats
- VernacularTitle:益赛普对脑外伤后核转录因子-κB、肿瘤坏死因子-α的表达及脑水肿的影响
- Author:
Tailing JI
;
Ailing DU
- Publication Type:Journal Article
- Keywords:
Traumatic brain injury;
Recombinant human tumor necrosis factor receptor Ⅱ-Fc fusion pro-tein;
Nuclear facter-κB;
Tumot necrosis factor-α
- From:
Clinical Medicine of China
2008;24(10):974-976
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of recombinant human tumor necrosis factor receptorⅡ-Fc fusion protein(rhTNFR:Fc ) on nuclear factor-κB(NF-κB)and tumor necrosis factor-α(TNF-α)protein expression in the rat brain tissue,and traumatic brain edema following acute traumatic brain injury(TBl).Methods The inju-ry rats were subjected to right lateral cortical impact injury caused by a free-falling object.In the rhTNFR:Fc group,rhTNFR:Fc was administered intraperitoneally(3.0 mg/kg)after 30 min of injury-and the rats of control and injury group administered with normal saline solution.The levels of TNF-α protein in rat brain were mensured by radio-im-munonssay(RIA).The NF-κB protein expression of rat brain was studied immunohistochemically.In the meantime,the water content of rat brain Was measured,and for the microscope and ultrastructure examinations by using electron-ic microscope respectively.Results Compared with control group,the levels of NF-κB and TNF-α protein and the water content of brain tissue were obvicously increased from 6 h following TBI(P<0.05,or P<0.01).Compared with injury group,the expression of NF-KB and TNF-α protein,and the brain water content were lower in rhTNFR:Fc group(P<0.05-P<0.01).The rhTNFR:Fc Can reduce the brain injury in electronic microscope examinations.Conclusion The expression of NF-κB and TNF-α,and the water content of rat brain increase significantly follow-ing acute traumatic brain injury.The rhTNFR:Fc can dramatically inhibit the expression of NF-κB and TNF-α,and alleviate rat brain edema after TBI.
