Efficiency comparison between two preimplantation genetic diagnostic methods for chromosomal translocation carriers
- VernacularTitle:荧光原位杂交技术不同方案用于染色体易位携带者胚胎植入前遗传学诊断的效率比较
- Author:
Yanwen XU
;
Xiulian REN
;
Ying LIU
;
Yanhong ZENG
;
Cong FANG
;
Ling GAO
;
Canquan ZHOU
;
Guanglun ZHUANG
- Publication Type:Journal Article
- Keywords:
Preimplantation diagnosis;
Translocation,genetic;
Heterozygote;
In situ hybridization,fluorescence
- From:
Chinese Journal of Obstetrics and Gynecology
2008;43(8):576-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the diagnostic efficiency between blastomere preimplantation genetic diagnosis (PGD) and polar body PGD for chromosomal translocation carriers. Methods Group A had 8 cycles using whole painting probes for the first polar body diagnosis, while group B had 29 cycles using two subtelomeric probes and one centromeric probe for the blastomere diagnosis. Results The fertilization rate of group A was significantly lower than group B [66. 1% (72/109) vs 85.2% (304/357) , P < 0.05]. There was no significant difference in the successful biopsy rate between two groups. However, group A had a significantly higher loss rate during fixation and higher no signal rate after fluorescence in situ hybridization [ FISH, 9. 6% (12/104) vs 1.6% (4/252), 11.2% (10/89) vs 3.0% (7/233) ]. Totally, the diagnostic efficiency in group A (72. 5% ,79/109 ) was significantly lower than that in group B( 89. 8%, 230/256, P < 0. 05 ). Although both the clinical pregnancy rate( 3/7 ) and implantation rate( 22. 2% ,4/18 ) of group A were higher, the differences were not statistically significant ( P > 0.05 ). Conclusion Both methods can be used efficiently in the PGD for chromosomal translocation carriers. Blastomere PGD has a higher diagnostic rate.
