Membrane-initiated nongenomic effect on mitogen-activated protein kinase signal transduction of estrogen in endometrial carcinoma cell
- VernacularTitle:雌激素通过子宫内膜癌细胞膜受体激活丝裂原活化蛋白激酶通路快速效应的初步研究
- Author:
Lili ZHANG
;
Xiaoping LI
;
Jianliu WANG
;
Lihui WEI
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Receptors,cell surface;
Estradiol;
Mitogen-activated protein kinase
- From:
Chinese Journal of Obstetrics and Gynecology
2008;43(8):615-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the nongenomic effect on mitogen-activated kinase (MAPK) signal transduction of 17β-estradiol at plasma membrane in endometrial cancer cell Ishikawa. Methods Estradiol-bovine serum albumin conjugates (E2-BSA) was added to Ishikawa cells at different time points. Western blot was used to detect the protein expression of phosphorylated extracellular signal-regulated kinase (ERK) 1/2. Confocal was used to verify if the phosphorylation of ERK1/2 was initiated at the membrane. Results Phosphorylation of ERK1/2 was elicited after 5 minute exposure to 1×10-7 mol/L E2-BSA. It was induced to a maximum following 60 minute exposure and descended after 2 hour. The expression levels of phospborylated ERK1/2 were 0. 52, 0. 98, and 0.09, respectively. Confocal proved that E2-BSA combined with membrane receptors after 5 minutes and elevated the phosphorylation of ERK1/2. Conclusion Membrane actions of estrogen can activate the rapid signaling cascades of MAPK in endometrial carcinoma cells.
