Effect of cyclic mechanical stimulation on expression of connective tissue growth factor in MG63 osteoblast-like cells
- VernacularTitle:机械应力对MG63成骨样细胞结缔组织生长因子表达的影响
- Author:
Min YANG
;
Liwei XIAO
;
Lingyun HUANG
;
Yiqun PENG
;
Guoliang SUI
;
Yuling HE
;
Rongrong CUI
;
Jiao HUANG
;
Ying LU
;
Eryuan LIAO
- Publication Type:Journal Article
- Keywords:
Osteoporosis;
Mechanical stimulation;
MG63 cells;
Connective tissue growth factor;
Mitogen-activated protein kinase
- From:
Chinese Journal of Endocrinology and Metabolism
2008;24(4):356-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate cyclic mechanical stimulation on expression of connective tissue growth factor (CTGF) in osteoblast-like cells (MG63) and to explore the rote of MAPK involved in the process.Methods Expressions of CTGF protein and mRNA in MG63 cells were detected by Western blot and RT-PCR,respectively. Phosphorylation levels of p38, ERK, JNK were examined by Western blot. Results Cyclic mechanical stimulation upregulated expressions of CTGF protein and mRNA. The levels reached a maximal response of 2-3 fold after 3-6 h. ERK and JNK signal pathways were activated by cyclic mechanical stimulation, the phosphorylated proteins increased within 10 min of stretch, phosphorylated ERK reached maximal levels by 60 min of stretch, phosphorylated JNK reached maximal levels by 15-30 min of stretch, but not for p38 signal pathway.Only the inhibitior of JNK signal pathway (SP600125) markedly suppressed stretch-induced CTGF expression,meanwhile the inhibitors of ERK (PD98059) and p38 (SB203580) did not show such effect. Conclusion Cyclic mechanical stimulation upregulates CTGF expression via JNK-dependent pathway in MG63 cells.
