Determination of the Bacillus probiotic strains used for biosubtyl production at IVAC by 168 rRNA gene sequences analysis and Western Blot
- Author:
Le Thi Loan
- Publication Type:Journal Article
- Keywords:
Bacterium
- MeSH:
Bacillus;
Blotting, Western
- From:Journal of Preventive Medicine
2005;15(6):70-75
- CountryViet Nam
- Language:Vietnamese
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Abstract:
The molecular technique for 168 rRNA gene sequences analysis and Western Blot were applied for determination of the Bacillus probiotic strains used in biosubtyl production at IVAC. The results showed that the nucleotide sequence identity of the NT strains was 99.74% in comparison with B. pumilus and the identity of the DL strains was 99.61% in comparison with B. cereus strains from the GenBank/EMBL/DDBJ Database. The result of Western blot analysis showed that, the antigen components of the strains NT and DL were not similar in their antigens, and they were not similar to the B. subtilis ATCC6633.
