Inhibitory effect of P195 and its antibodies on the invasion of merozoite of Plasmodium falciparum into human erythrocyte
- VernacularTitle:P195蛋白及其抗体抑制恶性疟原虫裂殖子入侵人红细胞的研究
- Author:
Jun FANG
;
Weibing GUAN
;
Feng QIAN
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum Protien 195 Antibody Erythrocyte
- From:
Chinese Journal of Infectious Diseases
1999;17(3):172-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To map out the binding site of P195,which is the major protein on the surface of P.falciparum merozoites,to human erythrocytes,and offer a basis for designing malaria vaccine to blockade invasion of merozoites into human erythrocytes.Methods Eight proteins derived from P195 were expressed in E.coli,and purified by Ni-chelare affinity chromatography.There after,the eight fragments and rabbit serums immunized by which were added into culture medium of P.fatciparum in vitro respectively.Twenty-four hours later,the invasion of merozoite to erythrocyte was observed.Results The antibodies which were induced by three fragments of P195,M6(Amino Acid,AA384～595),M7(AA 595～897)and M11(AA 1397～1563)could inhibit the invasion of P.falciparum merozoite into human erythrocytes.Especially,one fragment of P195,M6,had the ability to inhibit the invasion of P.falciparum merozoite into human erythrocytes.Conclusion M6,a fragment of P195 on the merozoite of P.falciparum may contain a domain thought to be involved in the recognition of human erythrocyte.The domain can be used as a candidate antigen for a malaria vaccine.
