Effects of recombinant human connective tissue growth factor on proliferation, differentiation, and apoptosis in human osteoblasts
- VernacularTitle:重组人结缔组织生长因子对人成骨细胞增殖、分化及凋亡的影响
- Author:
Guoliang SUI
;
Yiqun PENG
;
Yuling HE
;
Muxu ZHAI
;
Eryuan LIAO
- Publication Type:Journal Article
- Keywords:
Connective tissue growth factor;
Osteoblasts,human;
Osteoporosis;
Proliferation;
Apoptosis
- From:
Chinese Journal of Endocrinology and Metabolism
2008;24(4):360-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of recombinant human connective tissue growth factor (rCTGF) on the proliferation, differentiation and apoptosis in human osteoblasts. Methods Human osteoblasts were treated with rCTGF, cell proliferation was measured by [3 H] -thymidine ([3 H] -TdR) incorporation method,the activity of alkaline phosphatase (ALP) was determined using α-nitrophenyl phosphate as a substrate, the expression of type Ⅰ collagen was determined by Western blotting, osteocalcin in conditioned media was measured by radioimmune assay, and cell apoptosis was assayed by flow cytometry. Results rCTGF promoted proliferation and differentiation of human osteoblasts in a dose-depentent manner, rCTGF increased ALP activity, osteocalcin and type I collagen secretion in a dose-depentent manner, rCTGF inhibited human osteoblastic apoptosis induced by serum deprivation. Conclusion rCTGF can promote osteoblastic proliferation, and differentiation and protect osteoblast against apoptosis, suggesting that CTGF plays an essential role in bone metabolism.
