The expression of PPARγmRNA and protein in rats with acute necrotizing pancreatitis
- VernacularTitle:急性胰腺炎大鼠胰腺过氧化酶体增殖物激活受体的表达
- Author:
Jinping JIANG
;
Ping XU
;
Lingquan CHEN
;
Haiqing FAN
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Peroxisome proliferator-activated receptor γ;
Immunohistochemistry;
RT-PCR
- From:
Chinese Journal of Pancreatology
2008;8(4):250-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of PPARr mRNA and protein expressions in rats with ANP. Methods 36 rats were randomly divided into sham group and ANP group. The rats were sacrificed 3 h, 6 h,12 h after ANP induction, the levels of serum amylase were measured, the pancreatic pathological changes were determined and the expressions of pancreatic PPARr mRNA and protein was examined by RT- PCR and immunohistochemistry. Results In ANP group, the level of serum amylase at 6h was (7170.83± 1635.59) U/L, the scores of pathological changes were 6.67±1.03 and 13.00±2.36, which were much higher than those of sham group (P<0.01) ; the PPARr mRNA expression was 0.18±0.05, and there were no obvious differences compared with that of sham group (0.22±0.03 ) ; PPARr protein expression was 4.17 ±0.98, which was significantly higher than that of sham group (1.83±0.71, P<0.05). Conclusions Inflammatory injury resulted in increased deactivation of pancreatic acinar PPARr, meanwhile PPARr gene expression was inhibited by feedback.
