Effects of glucosides of chaenomeles speciosa on the expression of prostaglandins E2 receptor subtype EP2 and EP4 in synovial tissues of rats with adjuvant arthritis
- VernacularTitle:木瓜苷对佐剂性关节炎大鼠滑膜前列腺素受体表达的影响
- Author:
Liqin TANG
;
Wei WEI
;
Xiaoyu WANG
- Publication Type:Journal Article
- Keywords:
Adjuvant arthritis;
Synoviocytes;
Prostaglandins E2 receptors;
Glucosides of chaen-omeles speciosa
- From:
Chinese Journal of Rheumatology
2008;12(9):594-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of glucosides of cbaenomeles speciosa (GCS) on the expression of EP2 and EP4 in synovial tissues from rats with adjuvant arthritis. Methods Complete Freund's adjuvant (FCA) was used to induce adjuvant arthritis (AA) in rats. The synoviocytes from AA rats were collected and incubated. Synoviocytes proliferation was determined by 3-(4,5-dimethylthiazal-2yl) 2,5-dipbeny+hetrazoliumbromide (MTT) assay. Levels of prostaglandins E2(PGE2) and cAMP were determined by radioim-munoassay (RIA). The expression of EP2 and EP4 was detected by Western blot analysis. Results GCS significantly inhibited the proliferation of synoviocytes. GCS decreased the level of PGE2 and increased the level of cAMP. The results of correlation analysis demonstrated that GCS inhibited synoviocytes hyperplasia by increasing the level of cAMP in synoviocytes. The results of Western blot analysis demonstrated that GCS significantly improved the expression of EP2 and EP4. Condusion These results suggest that restoring normal transduction of the EP-G protein-cAMP signal pathway may be one of the most important mechanisms of GCS.
