Effect of tamoxifen on pituitary tumor transforming gene expression in C6 cell and tumor growth of glioma in vivo
- VernacularTitle:三苯氧胺对胶质瘤C6细胞垂体瘤转化基因表达及肿瘤生长影响的体内研究
- Author:
Da TENG
;
Ting WANG
;
Rufei DAI
- Publication Type:Journal Article
- Keywords:
Tamoxifen;
Glioma;
Pituitary tumor transforming gene
- From:
Journal of International Oncology
2008;35(8):634-636
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tamoxifen on pituitary tumor transforming gene ex-pression in C6 cell and tumor growth of glioma in vivo. Methods Animal models were established on 32 SD rats with C6 cells of glioma. The rats bearing with C6 glioma were divided into 4 groups randomly, which were treated without tamoxifen or with different doses of tamoxifen(0. 02 mg · kg-1 · d-1, 0. 2 mg · kg-1 · d-1, 2mg · kg-1 · d-1) once a day for 20 days. The dimension of tumors were measured, the tumor inhibition rates were caculated, and living state of the rats were observed. The expression of PTTG mRNA was detected by RTPCR. Results All kinds of doses of tamoxifen could inhibit the tumors growth in rats with C6 glioma, and the tumor volume were reduced by 47.6%, 35.5% and 21.2% in the high-, middle-and low-dose groups respec-tively, there were significantly differences among the 4 groups ( P < 0. 05 ). Low-, middle- and high- dose of tamoxifen all could inhibit the expression of PTTG mRNA, and there were significantly differences among the 4 groups in PTTG mRNA expression ( P < 0. 05 ). Conclusion Tamoxifen can inhibit PTTG expression and the growth of glioma cell line C6 in vivo significantly in dose-dependent manner, which provides a theory basis for clicinal use of tamoxifen in patients with gliomas.
