Fluorescence in situ hybridization analysis of HER-2/neu oncogene amplification in osteosarcomas
- VernacularTitle:应用荧光原位杂交技术检测骨肉瘤患者人表皮生长因子2受体基因扩增状态
- Author:
Jian SHANG
;
Wenbo WANG
;
Guofu YANG
- Publication Type:Journal Article
- Keywords:
Epirnd growth factor receptor genes;
Osteosarcoma;
Fluorescence in situ hy- bridization;
Gene amplification
- From:
Chinese Journal of Orthopaedic Trauma
2008;10(9):867-869
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether HER-2/neu oneogene amplification exists in 08- teosarcomas. Methods Fluorescence in situ hybridization(FISH)and DNA probes for HER-2/neu and centromere 17 were used to examine the HER-2/neu gene amplification in samples taken from 23 patients with osteosarcoma. Results None of the 23 osteosarcoma samples showed the HER-2/neu gene amplification.Condusion Since HER-2/neu gene amplification is rare in osteosarcomas, HER-2/neu target medicine should not be applied to patients with osteosarcoma
