Effect of sinomenine on the expression of TNF-α-induced vascular cell adhesion molecule 1 on human umbilical vein endothelial cells
- VernacularTitle:青藤碱对肿瘤坏死因子-α诱导人脐静脉内皮细胞VCAM-1表达影响初步研究
- Author:
Jianlin HUANG
;
Yuqiong WU
;
Minqi LUO
;
Qiujing WEI
;
Buyun YU
;
Jieruo GU
- Publication Type:Journal Article
- Keywords:
Sinomenine;
Vascular cell adhesion molecule 1;
Human umbilical vein endothelial cells
- From:
Chinese Journal of Rheumatology
2008;12(9):613-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective To further investigate the effect of sinomenine (SIN) on TNF-α-induced VCAM-1 expression in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were isolated from freshly collected umbilical cords. Positive control samples were stimulated with TNF-α, but free of SIN. Negative control samples were treated in the same way, but without TNF-α and SIN. Experimental samples were co-cultured with TNF-α and SIN at various concentrations (0.25, 0.5, and 1.0 mol/L), or TNF-α and dexamethasone (Dex) at concentration of 1.0×10-6 mol/L, or TNF-α with Dex (at concentration of 1.0×10-6mol/L) and SIN at different concentrations (0,25, 0.5, and 1.0 mmol/L) (co-treated groups). VCAM-1 expression was detected by flow cytometry (FCM). Results SIN inhibited expression of VCAM-1 in TNF-α-induced HUVECs, the best effect was shown in the 1.0 mmol/L SIN treated group. VCAM-1 decreased more markedly in the co-treated groups. Conclusion SIN inhibits TNF-α-induced VCAM-1 expression on HUVECs in vitro, and SIN maybe synergistic with Dex in inhibiting TNF-α-induced VCAM-1 expression on HUVECs in vitro.
