Expression of FOXO1 and FOXO3a on peripheral blood mononuclear cells and their clinical roles in patients with systemic lupus erythematosus
- VernacularTitle:FOXO1和FOXO3a在系统性红斑狼疮患者外周血单个核细胞中的表达及其与疾病活动性的关系
- Author:
Dongyu CHEN
;
Zhaofeng SONG
;
Fang LI
;
Deqing ZHANG
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Transcription factor;
Peripheral blood mononuclear cell;
FOXO1;
FOXO3a
- From:
Chinese Journal of Rheumatology
2008;12(9):629-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of transcription factor FOXO1 and FOXO3a on peripheral blood mononuclear cells (PBMC) in patients with active or inactive systemic lupus erythematosus (SLE) and investigate the effect of FOXO1 and FOXO3a on the clinical features of SLE. Methods Thirty SLE patients and 10 healthy controls were enrolled. PBMC were separated from the peripheral blood. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot were applied to analyze the expression of FOXOI and FOXO3a. Results The level of FOXO1 expression was significantly decreased in active SLE patients compared with controls and patients with inactive SLE (P<0.05). The level of FOXO1 expression in inactive SLE patients was lower than that of the controls (P<0.05). The expression of FOXO1 mRNA was negatively correlated to SLEDAI. However, the level of FOXO3a was similar among the three groups. Conclusion The result suggests that FOXO1 may be involved in the pathogenesis of SLE and the expression level of FOXOI may be a good indicator for the disease activity of SLE.
