Effects of Sinomenine on the rat dendritic cells after hepatic ischemia-reperfusion
- VernacularTitle:青藤碱对大鼠肝脏缺血再灌注后树突状细胞功能的影响
- Author:
Feng LIN
;
Wenyuan GUO
;
Ning MU
;
Hong FU
;
Zhengxin WANG
;
Guoshan DING
;
Zhiren FU
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Ischemia-reperfusion injury;
Sinomenine;
Dendritic cell
- From:
Chinese Journal of Digestive Surgery
2008;7(5):360-362
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Sinomenine on the dendritic cells after hepatic ischemia-reperfusion. Methods Forty-eight BN rats were equally divided into control group, low dose (40 μg/g) and high dose (80 μg/g) of Sinomenine groups after the liver transplantation models were established by two cuff tech-nique. Three days after the orthotopic liver transplantation, the livers were resected, then the dendritic cells were separated and purified. The phenotypes [OX62, major histocompatibility complex Ⅱ (MHC-Ⅱ) and CD86] of dendritic cells were examined by FACS, the expression of IL-12, IL-1, and TNF-a mRNA by RT-PCR, and the expression of Toll-like receptor 4 (TLR4) by Western blot. Results The dendritic cells treated with Sinomenine showed immature phenotypes. The expressions of MHC-Ⅱ and CD86 were significantly deceased. The expressions of IL-12, IL-1, TNF-a mRNA and TLR4 were low. Conclusions Sinomenine can significantly inhibit the maturity and immunologic function of dendritic cells after hepatic ischemia-reperfusion.
