Therapeutic effect of NgR siRNA on nerve regeneration following spinal cord hemi-transsection injury in rats
- VernacularTitle:Nogo-66受体单链RNA干扰对大鼠脊髓半切损伤的治疗效应
- Author:
Yinghui BAO
;
Jianhong SHEN
;
Yumin LIANG
;
Qizhong LUO
;
Jiyao JIANG
- Publication Type:Journal Article
- Keywords:
Spinal cord injury;
Receptor,Nogo protein;
RNA interference
- From:
Chinese Journal of Trauma
2008;24(9):696-699
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of NSR siRNA on nerve regeneration following spinal cord hemi-transsection injury in rats. Methods Rats with T8 spinal cord hemi-trans-section were didded into 3 groups, ie, siRNA group, NS group and control group. SiRNA or NS was in-jected into lateral cerebral ventricle just after spinal cord injury. The therapeutic effect of NgR siRNA was evaluated by using BBB locomotor rating scale, retrograde horseradish peroxidase(HRP)tracing and HE staining. Results BBB locomotor rating scale showed that the recovery of the locomotor function of siRNA group seemed to be better than that of the other two groups from the 4th week, but there was no statistical difference. Retrograde HRP tracing showed a large number of positive cells in the anterior horn of spinal cord, with statistical difference compared with NS group and control group(P<0. 05). Eight weeks after spinal injury, HE staining showed disorderly distribution of the fibres in NS group and control group but serial fibres in the injury region in siRNA group. Conclusion NSR siRNA may promote the nerve regeneration following spinal cord injury.
