Establishment of a rat model of bone cancer pain induced by intra-tibia inoculation of Walker 256 mammary gland carcinoma cells
- VernacularTitle:大鼠胫骨内接种Walker256乳腺癌肉瘤细胞建立骨癌痛模型
- Author:
Jingxiang WU
;
Xuerong MIAO
;
Xiaoqing LI
;
Meiying XU
;
Weifeng YU
- Publication Type:Journal Article
- Keywords:
Disease models,animal;
Bone neoplasms;
Pain
- From:
Chinese Journal of Anesthesiology
2008;28(8):691-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat model of bone cancer pain by intra-tibia inoculation of Walker 256 mammary gland carcinoma cells. Methods Sixty female Wistar rots weighing 180-200 g were randomly divided into 4 groups (a=15each):groupⅠ normal control; group Ⅱsham operation; group Ⅲtumor cell inoculation + normal saline (NS) and group Ⅳtumor cell inoculation + flurbiprofen. NS 0.2 nd and flurbiprofen 10 mg/kg in 0.2 ml were injected IV at 2 h before determination of pain threshold on 14 and 17 d after inoculation oftumor cells in groupⅢand Ⅳ respectively. On day 0, 4, 7, 10, 14, 17 and 21 after inoculation pain threshold was measured after determination of body weight. X-ray examination of the tibia was performed on day 14 after inoculation. The animals were killed on day 21 after inoculation for microscopic examination of the inoculated tibia. Results The animals started losing weight and the threshold to yon Frey hair stimulation was decreased from dhy 10 after inoculation in group Ⅲand Ⅳ. X-ray examination showed destruction of bone and microscopic examination showed tumor growing in tibia. Flurbiprofen significantly decreased mechanical hyperalgesia in group Ⅳ. There was no significant difference in paw withdrawal latoney to radiant heat among the 4 groups. Conclusion A model of bone cancer pain can be made by inoculation of Walker 256 mammary gland carcinoma cells into tibia characterized by mechanical hyperalgesia.
