The effect of KLT on apoptosis of HepG2 cells and expression of Bcl-2 and Caspase-8
- VernacularTitle:康莱特诱导肝癌细胞HepG2凋亡及其对Bcl-2、Caspase-8表达影响的研究
- Author:
Baolei WANG
;
Xin JIN
;
Changsheng LI
;
Yun LU
- Publication Type:Journal Article
- Keywords:
KLT;
Bcl-2;
Caspase-8;
HepG2 cell;
apoptosis
- From:
International Journal of Surgery
2008;35(10):666-669
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of KLT on apoptosis of HepG2 cells and expression of Bcl-2 and Capase-8. Methods The cell fine HepG2 was induced by diverse density of KLT, and HepG2 cell was collected respectively after induction of 12 h, 24 h, 48 h. The control group was installed simulta-neon]y. The cell apoptosis and the expression of Bcl-2 and Caspase-8 were detected by flow cytometry (FCM). Results KLT can induce the apoptosis of HepG2 cell significantly, and the longer time past, the more apoptosis of HepG2 was. KLT can increase the expression of Caspase-8, but ineffective to Bcl-2. Con-clusion KLT can significantly induce the apoptosis of HepG2 cell through regulating the expression of Caspase-8.
