Expression of platelet-derived growth factor and its receptor at human and mouse neuromuscular junctions
- VernacularTitle:血小板衍生生长因子及其受体在人及小鼠神经肌肉接头的表达研究
- Author:
Yajuan ZHAO
;
Xiaolin SU
- Publication Type:Journal Article
- Keywords:
Platelet-derived growth factor;
Receptor;
Neuromuscular junction
- From:
Chinese Pediatric Emergency Medicine
2008;15(5):442-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunoreactivity of platelet-derived growth factor-A (PDGF-A) and its receptor at human and mouse neuromuscular junctions and to explore their regulatory effects on neuromuscular junctions. Methods Specific polyclonal antibodies were used to detect PDGF-A and PDGF a-receptor expression. Double immunohistochemistry for platelet-derived growth factor and the acetylcholine receptor was performed on normal human muscle biopsy specimens. Double fluorescence labeling was applied to detect immunoreactivity for PDGF-A, its receptor and acetylcholine receptors. Results PDGF-A and its receptor was closely co-localized with acetylcholine receptors at human and mouse neuromuscular junctions. Conclusion PDGF-A and its receptor concentrated at human and mouse neuromuscular junctions. PDGF might be involved in the interaction between the presynaptic and postsynaptic components, PDGF-A and its receptor might play regulatory role in signaling at neuromuscular junctions in normal muscle.
