Effect of Mycophenolic Acid on the proliferation of pulmonary arterial smooth muscle cells in rats
- VernacularTitle:霉酚酸对大鼠肺动脉平滑肌细胞增殖的影响
- Author:
Xuhua SHI
;
Yi ZHENG
;
Hongying ZHAO
;
Mulan JIN
- Publication Type:Journal Article
- Keywords:
Myeophenolic acid;
Rat;
Myocytes,smooth muscle;
Pulmonary artery
- From:
Chinese Journal of Rheumatology
2008;12(10):673-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mycophenolic acid on the proliferation of smooth muscle cells (SMCs) in rats' pulmonary arterial at cellular level. Methods Growth curve, MTT tests, and flow cytometry were used to detect the number of proliferative cells, the A-value of living cells and the DNA content of the control and drugs groups respectively and the number of cells in G1-phase, S-phase, G2M- phase and the proliferation index were calculated. Results Compared with the control group, the number of living cells in the mycopbenolic acid groups (with the concentration of 1, 10, 100 μmol/L) decreased, except the lowest dosage group (100 nmol/L). The difference was statistically significant (P<0.01). The living cells measured by MTT dose-dependently reduced in the mycophenolic acid groups. In the mycophenolic acid groups, the fraction of living cells in the S-phase and G2M-phase decreased respectively while that in G1- phase increased, and the proliferation index decreased. All these responses presented with a dose-dependent manner. Conclusion Mycophenolic acid can effectively inhibit the proliferation of rats' pulmonary arterial SMCs. This process happens mainly in ONA synthesis phase, and is dose dependent. In addition, these effective concentrations are all in the available range for clinical application.
