The comparison of two different detection methods for anti-gp210 antibody
- VernacularTitle:人工合成gp210多肽抗原在原发性胆汁性肝硬化中的应用
- Author:
Lixia GAO
;
Fengchun ZHANG
;
Bin LIU
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis,biliary;
Enzyme-linked immunosorbent assay;
gp210 antigen;
Anti-gp210 antibody
- From:
Chinese Journal of Rheumatology
2008;12(10):677-679
- CountryChina
- Language:Chinese
-
Abstract:
Objective To attempt using the synthetic poly-peptides in the carboxyl terminal of gp210 antigen as the substrate for anti-gp210 antibody detection and search for a simple assay method of detecting anti-gp210 antibody. Methods The enzyme linked immunosorbent assay (ELISA) method for anti-gp210 antibody detection was set-up by chessboard test. Anti-gp210 antibody was tested in the serum of both patients with primary biliary cirrhosis (PBC) and the control group by ELISA and immuno-blotting. Results The working concentration of gp210's antigen was 5 μg/ml. The optical density higher than 0.61 (x+3s) was designated as the positive cutoff of anti-gp210 antibody. There was no statistical difference between the two assays (P=0.617). And there was very strongly positive correlation between them (P=0.000, r=0.868). Conclusion The sensitivity and specificity are basically consistent between the assays using synthetic poly-peptides of gp210 antigen and native antigen as the detecting substrates. The former substrate is preferred to be used for the testing of anti-gp210 antibody in clinical laboratories.
