Expression of toll-like receptor on different cell surface in PMN recruiting in severe acute pancreatifis
- VernacularTitle:不同细胞表面TLR4表达对小鼠重症急性胰腺炎中性粒细胞招募的作用
- Author:
Junwei BAO
;
Heshui WU
;
Chunyou WANG
;
Jingxiang ZHANG
;
Jinghui ZHANG
;
Yuan TIAN
;
Ying XU
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Toll-like receptor 4;
Endotoxins;
Transplantation chimera
- From:
Chinese Journal of General Surgery
2008;23(10):756-759
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of expression of toll-like receptor on medullary system cell and endothelial cell surface in PMN recruiting in severe acute pancreatitis.Method In this study,26 C3H/He-J mice and 26 C3H/He-N mice were derided into 4 groups by bone marrow transplantation:group A, mosaic of medullary system cell TLR4-/- and endothelial cell TLR4+/+,group B, mosaic of medullary system cell TLR4+/+ and endothelial cell TLR4-/- ;group C, homozygote ofTLR4+/+ ;group D, homozygote of TLR4-/-. SAP was induced to all these mice. Amylase, TRIA of granular cell in peripheral blood,TRL4 expression in pancreas, Naphthol AS-D chloroacetate esterase, MPO activity were tested. Results Amylase level increased in all mice, there was no differences of the amylase level among groups, TRIA of granular cell in peripheral blood in group B and C was significant higher than A and D(P<0.05), TLR4 in pancreas were positive in group A and C, negative in group B and D, AS-D count and M PO activity in group A and C were higher than that in group B and D (P < 0. 05 ). Conclusions It was endothelial cell, not peripheral blood granulocyte, that plays a key role in recuiting neutrophils that triggers severe acute pancreatitis.
