Influence of Keloid-derived Keratinocyte on TGF- beta1 Production of Fibroblast in Co-culture Model.
- Author:
Jun PARK
1
;
Sang Yoon KANG
;
Young Cheun YOO
;
Won Yong YANG
Author Information
1. Department of Plastic and Reconstructive Surgery, College of Medicine, Kyung Hee University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Keloid;
TGF-beta1
- MeSH:
Biology;
Cicatrix;
Coculture Techniques*;
Collagen;
Enzyme-Linked Immunosorbent Assay;
Fibroblasts*;
Keloid;
Keratinocytes*;
Membranes;
RNA, Messenger;
Skin;
Transforming Growth Factor beta1
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2004;31(4):554-562
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Keloids represent a pathological response to injury interrupting skin integrity, creating disfiguring scars with no definitive pathophysiology. Transforming growth factor-beta1(TGF- beta1) has been regarded as one of the pathogenesis of keloids. The purpose of this study is to investigate the influence of keloid keratinocytes on TGF-beta1 expression in co-culture system. Recent studies have highlighted the important concept of epithelial- mesenchymal interactions in normal skin biology, and applying this concept to keloids in vitro studies demonstrated significantly increased proliferation of fibroblasts and collagen production in keloid fibroblasts co-cultured with keloid-derived keratinocytes as compared with normal keratinocytes. In this study, normal or keloid fibroblasts in lower chamber were co-cultured with keratinocytes derived either from normal skin or keloid tissue in upper chamber, separated from lower chamber by permeable membrane. Results obtained by ELISA showed equal TGF-beta1 expression when keloid fibroblasts were co- cultured with keloid keratinocytes, as compared with the normal keratinocytes at 24,48 hours, but significantly higher expression at 72 hours(p=0.0358). RT-PCR demonstrated that the TGF- beta1 expression level was not significantly related to the level of mRNA encoding TGF-beta1. These results suggest that overlying keloid keratinocytes play an important role in inducing high level of TGF- beta1 expression in keloid by paracrine effect or epithelial-mesenchymal interaction.
