Survivin expression in endometriosis and normal human endometrium and effects of GnRH-a and COX-2 inhibitor on its expression.
- VernacularTitle:生存素基因在子宫内膜异位症中的表达及促性腺激素释放激素激动剂、环氧合酶-2抑制剂对其表达的影响
- Author:
Zhiqun ZHENG
;
Xiaoqing LIU
- Publication Type:Journal Article
- Keywords:
Endometriosis;
Survivin;
In vitro cell culture;
GnRH-a;
COX-2 inhibitor
- From:
Clinical Medicine of China
2008;24(11):1135-1137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of survivin gene in the pathogenesis of endometriosis (EMs). Methods The expressions of survivin in endometriosis and normal endometrium tissue were determined ; the effects of GnRHa and COX-2 on the expression of survivin mRNA in endometriosis and normal endometrium in vitro and the effects of GnRHa and COX-2 on the apoptosis index in the cultured ectopie endometrial cells were investigated. Re-sults ①The expression of survivin mRNA was higher in patients with endometriosis than that of healthy controls (P <0.01) ,with no cyclical variation. ②GnBHa exerted a dose-dependent suppression of survivin mRNA expres-sion in cultured ectopic endometrioma cells as well as COX-2. The significant suppression was observed at the 100μg/L concentration of GnRHa and at the 40 μmol/L concentration of COX-2. No cooperation was found between them (P > 0.05). Conclusion ①The up-regulation of survivin mRNA expression may reduce the sensitivity of en-dometriotic cells to apeptosis. Elevated expression of survivin mRNA in ectopic endometrium may have important im-plications for the survival and proliferation of the ectopic endometrial tissue.②Both GnRHa and COX-2 can promote apoptosis by inhibiting survivin mRNA expression in ectopic endometrioma cells in vitro.
