The expression of CD40 and CD40L on the surface of peripheral blood mononuclear cells in asthmatic rats and the effect of antiCD40L McAb on Th1 and Th2 cytokines
- VernacularTitle:哮喘大鼠外周血单个核细胞CD40、CD40L表达和CD40L单克隆抗体对Th1、Th2细胞因子生成的影响
- Author:
Keying XUE
;
Shengdao XIONG
;
Weining XIONG
;
Li CHENG
;
Chengguo WANG
;
Wei LI
;
Ming SHI
- Publication Type:Journal Article
- Keywords:
Asthma;
Antigens,CD40;
CIM0 ligand;
Antibodies,monoclonal;
Intefleukin-4;
Interferon type Ⅱ
- From:
Journal of Chinese Physician
2008;10(9):1173-1175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CD40 and CD40L on the surface of peripheral blood mononuclear cells(PBMCs)in asthmatic rats and the effect of anti-CD40L McAb on cytokines of it.Methods Flow cytometry and RT-PCR were used to detect the expression of CD40 and CD40L of PBMCs ih asthmatic rats.After the PBMCs Was treated with anti.CIMOL McAb.ELISA was used to detect the levels of IL-4 and IFN-γin the supematants of cultured cells.Results Compared with the normal control group.the expression of CD40 and CD40L of PBMCs in asthImatic rats increased(P＜0.05).Compared with the untreated group,the level of IL-4 and the ratio of IL4/IFN-γ decreased after the PBMCs were treated with anti-CD40L McAb(P＜0.05).Conclusion The expression of CD40 and CD40L on the surface of PBMCs in asthmatic rats Was unregulated.Anti-CD40L Mcab Can decrease the level of IL-4 and the ratio of IL_4/IFN-γ.
