In vitro induction,proliferation and function characterization of dendritic cells from rat bone lnRITOW cells
- VernacularTitle:大鼠骨髓来源树突状细胞的体外诱导扩增及其功能鉴定
- Author:
Nan WANG
;
Qingjiu MA
;
Jianguo LU
;
Xianli HE
;
Na LI
;
Rui DONG
;
Jikai YIN
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Bone marrow;
Granulocyte-maerophage colony-stimulating factor;
Interleukin-4
- From:
Journal of Chinese Physician
2008;10(9):1176-1179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of inducing dendritic cells(DC)from rat bone marrow cells in vitro,and identify the phenotype and function characteristics.Methods The rat bone malToW cells were collected and cultured in vitro under the condition of recombinant rat GM-CSF(rrGM-CSF)and recombinant rat IL-4(rrIL-4).After 2 weeks,the morphological character of DCs was observed under light microscope and scanning electron microscope.Expression of MHC-Ⅱ,CD80 and CD86 were detected by flow cytometry.The ability to stimulate allogenic T cells of the cultured DCs was detected by mixed lymphocyte reaction.Results DCs showed typical morphology with elongated dendritic processes under inversion microscope and scanning electron microscope.DCs at day 6 revealed immature phenotype,including MHC-Ⅱ(29.03 ±4.39)%,CD80(21.98±7.08)%and CD86(25.94±6.80)%.DCs at day 12 showed higher expression of MHC-Ⅱ(74.05±5.97)%,CD80(79.85±6.53)%and CD86(81.00±7.47)%,and stimulatory capacity of allogenic T cells,compared with that in DCs at day 6.Conclusion Matured DCs could be generated from rat bone marrow cells and attendance with rrGM-CSF and rrIL-4,which present the feasibility for further research on its application to allograft immunorejection.
