The Effects of Irradiation and Calcium-deficient Diet on the Expression of Interleukin-1 during Tooth Formation of Rat Molar.
- Author:
Il Joong KIM
1
;
Eui Hwan HWANG
;
Sang Rae LEE
Author Information
1. Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University, Korea. hehan@khu.ac.kr
- Publication Type:Original Article
- Keywords:
irradiation;
calcium-deficient diet;
interleukin-1;
tooth formation
- MeSH:
Abdomen;
Animals;
Atrophy;
Blood Vessels;
Collagen;
Dental Cementum;
Dental Sac;
Diet*;
Fibroblasts;
Interleukin-1*;
Maxilla;
Microscopy;
Molar*;
Osteoblasts;
Osteoclasts;
Periodontal Ligament;
Pregnancy;
Rats*;
Tooth Germ;
Tooth*
- From:Korean Journal of Oral and Maxillofacial Radiology
2000;30(3):159-168
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To elucidate the effects of the irradiation and calcium-deficient diet on expression of interleukin(IL)-1 during tooth formation of rat molar MATERIALS AND METHODS: The pregnant three-week-old Spague-Dawley rats were used for the study. The control group was non-irradiation/normal diet group, and the experimental groups were irradiation/normal diet group and irradiation/calcium-diet group. The abdomen of the rats on the 9th day of pregnancy were irradiated with single dose of 350 cGy. The rat pups were sacrificed on the 14th day after delivery and the maxillae tooth germs were taken. The specimen were prepared to make sections for light microscopy, and some of tissue sections were stained immunohistochemically with anti-IL-1 antibody. RESULTS: In the irradiation/normal diet group, dental follicle showed fewer blood vessels, mononuclear cells, and fusions of mononuclear cells than in non-irradiation/normal diet group. Alveolar bone showed a few osteoblasts and osteoclasts. Periodontal ligament showed collagen fibers and fibroblasts with irregularity. Weak immunoreactivity for IL-1 was shown in dental follicle, alveolar bone, and periodontal ligament. In the irradiation/calcium-deficient diet group, dental follicle showed sparse cellularity. Alveolar bone showed diminished number of osteoblasts. Periodontal ligament showed irregular collagen fibers and atrophy of cementoblasts and fibroblasts. No immunoreactivity for IL-1 was shown in dental follicle, alveolar bone, and periodontal ligament. CONCLUSION: Irradiation and calcium-deficient diet seems to cause disturbance of the expression of interleukin-1 during tooth formation of rat molar.
