Research progress in visualizing single molecule in live cells
- VernacularTitle:活细胞内单分子视踪检测研究进展
- Author:
Deping SUN
;
Kai YANG
- Publication Type:Journal Article
- Keywords:
Live cells;
Single molecule;
Visualizing
- From:
International Journal of Biomedical Engineering
2008;31(5):272-276
- CountryChina
- Language:Chinese
-
Abstract:
Our knowledge of molecular events and interactions in cell biology and molecular biology are mostly based on findings in nonliving body condition while ignoring the whole process of the events,which inevitably involves false assumption that all molecules behave in the same environment and in the same way. With the emergence of new fluorescent molecular probes prepared through linking new fluorescent material such as quantum dots (QDs) to micromolecule antibody such as antibody simulacrum, the development of methods for transducing extrinsic fluorescent probes into live cells,the application of laser technology, the finer fluorescent micro-imaging system, and the fast and sensitive fluorescence collection system in the application of life sciences, it is now possible to image the trajectory, kinetic behavior, and interaction of biomolecules in live cells. Visualizing single molecule in live cells has opened a new chapter for cell biology and molecular biology. Recent research developments and foreground of single molecule visualizing are discussed in this article.
