Exogenous wild type p53 gene affects radiosensitivity of human lung adenocarcinoma cell line under hypoxia
- VernacularTitle:乏氧状态下外源性野生型p53基因对肺癌细胞照射敏感性的影响
- Author:
Jianhua WANG
;
Feng WANG
;
Yongping LIU
;
Shirong LI
;
Yaping ZHANG
;
Yan NI
- Publication Type:Journal Article
- Keywords:
Cell line,tumor;
Lung adenoearcinoma,human;
Genes,p53;
Hypoxia;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2008;17(6):470-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of exogenous wild type p53 (wtp53) gene on radiosensitivity of human lung adenoeareinoma cell line under hypoxia. Methods Human lung adenoeareinoma cell line A549 was transfected with adenovirus carrying recombinant exogenous wtp53. Four irradiation groups were studied : normal cell ( Group A), wtp53 transfeeted cell ( Group B ), normal cell under hypoxia (Group C) and wtp53 transfected cell under hypoxia( Group D). Ceils were irradiated with 9 MeV electron beams. Cellular survival fraction was analyzed. Multi-target single-hit model was used to plot the survival curve. D0,Dq ,oxygen enhancement ratio(OER) ,sensitizing enhancement ratio(SER) and other parameters were used to evaluate the effects of wtp53 gene on radiosensitivity of A549. The cell apoptotie rate of each group was examined by flow eytometry. Results OER was 1.75 and O. 81 before and after wtp53 transfeetion. SER was 1.77 in oxic circumstance and 3.84 under hypoxia. The cell apoptotie rate of Group A and B was lower than Group C and D(F=7.92 ,P=0.048) ,with Group A lower than B and Group C lower than D (F=82.50,P=0.001). But Group B and D were similar(t=2.04,P=0. 111). Conclusions Hypoxia can increase the radiation resistance of lung adenoeareinoma cell line .4,549. The wtp53 can promote apoptosis and improve tumor radiosensitivity,especially under hypoxia.
