Effects of RNA interference-mediated silencing expression of survivin gene on invasion of human prostate cancer cell
- VernacularTitle:RNAi抑制存活素基因表达对前列腺癌细胞侵袭的影响
- Author:
Yu FAN
;
Youli ZHANG
;
Hua LI
- Publication Type:Journal Article
- Keywords:
Prostate carcinoma;
Neoplosm invasiveness;
Matrix metalloproteinases
- From:
Chinese Journal of Geriatrics
2008;27(11):847-850
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effects and mechanism of survivin gene on invasion of prostate cancer cell.MethodsAfter PC-3 prostate cancer cell lines were transfected by survivin small interfering RNA (siRNA), the mRNA and protein of survivin and matrix metalloproteinase-2 and-9 were determined by real time RT-PCR and western blot assay, respectively. The anchorage-independent growth was examined by clon formation in soft agar, and invasion ability was evaluated by boyden chamber model.The invasion ability of cancer cells in vivo was determined by nude mice model. ResultsThe results from clon formation in soft agar showed that the colonies numbers of group of 3.125,6.250 and 12.500 nmol/L of siRNA were 17.8±1.6,13.6±1.5 and 8.8±1.4, and the control group was 22.6±1.8(P<0.05). The results from boyden chamber model exhibited that the cells numbers crossing filter membrane in group of 3.125,6.25 and 12.5 nmol/L of siRNA were 33.6±2.1,19.5±1.9,8.1±1.83, and the control group was 49.4±2.3(all P<0.05). The results in vivo showed that cancer cells of control groups invaded into striped muscle and blood vessel, and there were no these phenomenons in transferred group with survivin siRNA. Survivin siRNA could reduce expression level of MMP-2 and MMP-9 in prostate cancer cells(P<0. 01).Conclusions Survivin-directed RNA interference can inhibit invasion of human prostate cancer cell through down-regualting MMP-2,-9 genes.
