In vitro expansion of hematopoietic stem/progenitor cells from human umbilical cord blood.
- VernacularTitle:骨髓间充质干细胞支持脐血造血干/祖细胞体外扩增的研究
- Author:
Ying GAO
;
Hongnv CHU
;
Chunjue GUO
;
Meijue SHEN
;
Xiaoling LV
;
Yuning HOU
;
Jinfu WANG
- Publication Type:Journal Article
- Keywords:
Bone marrow cells/CY;
Mesenchymal stem cells/CY;
Cell culture;
Hematopoietic stem cells;
Cell proliferation
- From:
Journal of Chinese Physician
2008;10(10):1303-1306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To separate and cultivate homo umbilical cord blood (UCB) hematopoietic stem cell (HSC) in vitro, and u-tilize bone marrow desmohemoblast stem cell as trophoblastic layer combined with cytokine to amplify umbilical cord blood hematopoietic stem/progenitor cell. Methods Ficoll lymph-cell separating medium density gradient centrifugalization was used to segregate UCBHSC.Bone marrow desmohemoblast stem ceil and cytokine were added, and the sum of NC cells and CD34 + cells was counted. Results The sum of NC cells amplified 75.2±15.0 times, and the sum of CD 34 + cells amplified 18.7±12.3 times. Conclusions It has significant effect on amplification of hematopoietic stem cell with bone marrow desmohemoblast stem cell and eytokine when HSC are cultured in vitro.
