Detection of 11q23 deletion and trisomy 12 in chronic lymphocytic leukemia by interphase fluorescence in situ hybridization
- VernacularTitle:间期荧光原位杂交技术检测慢性淋巴细胞白血病12三体和11q23缺失
- Author:
Yuexin CHENG
;
Mingfang CHEN
;
Wei XU
;
Tianrong CHEN
;
Li LI
;
Qigen SHEN
;
Jianyong LI
- Publication Type:Journal Article
- Keywords:
Leukemia,lymphocytic,ehronic/GE;
Chromosome deletion;
In situ hybridization,fluorescence
- From:
Journal of Chinese Physician
2008;10(10):1297-1299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the incidence of trisomy 12(+12) and 11 q23 deletion [ del ( 11q23) ] in chronic lymphocyticleukemia (CLL). Methods Fluorescein labeled DNA probe 12 and sequence specific probe ATM for 11q23 were used to perform inter-phase fluorescence in situ hybridization (I-FISH) assays in 30 patients with CLL. The results were compared with that of conventional cyto-genetic (CC) examination. Results With CC examination , only 4 cases (13.3%) were found to have chromosomal abnormalities, whereaswith I-FISH assay ,8 cases (26.7%) were found to have genomic aberrations, including trisomy 12 in 5 cases , deletion of 11q23 in 3 ca-sea. Conclusion I-FISH is a useful method for detection of genomie aberration in CLL, the significance of trisomy 12 and del (11q23) inpredicting the prognosis of B-CLL need to be investigated further.
