Establishment and clinical application of fluorescent polymerase chain reaction for the determination of-88/-123 polymorphisms in the Myxovirus resistance protein A gene promoter
- VernacularTitle:人MxA基因启动子-88/-123位多态性荧光PCR检测方法的建立及临床应用
- Author:
Jie YU
;
Weimin MA
;
Xia LONG
;
Lijia CHEN
;
Junmei HUANG
;
Yanzhong PENG
;
Jiazhi FANG
- Publication Type:Journal Article
- Keywords:
Proteins Antiviral agents;
Operon;
Polymorphism,genetic;
Interferon alfa-2b
- From:
Chinese Journal of Infectious Diseases
2008;26(10):580-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a fluorescent polymerase chain reaction (PCR) method for rapid, sensitive and specific determination of -88/-123 polymorphisms in Myxovirus resistance protein A (MxA) gene promoter so as to provide molecular biology tool for optimized interferon-a treatment in chronic hepatitis B patients. Methods Hepatitis B virus (HBV) genotyping,serum HBV DNA level,and- 88/- 123 polymorphisms in MxA gene promoter of patients who had been treated with interferon-α were detected. The statistical analysis was done by using SPSS software to understand the relationship between MxA gene polymorphisms and interferon-α treatment. Afterwards, an optimal fluorescent PCR system was established to determine -88/-123 polymorphisms in MxA gene promoter. The sensitivity and the specificity of this system were confirmed by DNA sequencing. P-value of chi square test, odds ratios of regression analysis and 95% confidence intervals were employed. Results Patients with- 88 G/T and - 123 C/A in the interferon-stimulated response element in MxA gene promoter were interferon-α sensitive, while patients with - 88 GIG and - 123 C/C were not interferon-α sensitive. The coincidence rate of this system was 99.65% in comparison with DNA sequencing.Conclusion MxA gene polymorphisms could be rapidly and sensitively determined by this fluorescent PCR system.
