Preparation of bovine serum albumin nanoparticles loaded with gemcitabine and the cytotoxic effect on pancreatic cancer cells in vitro
- VernacularTitle:吉西他滨白蛋白纳米粒的制备及其对胰腺癌细胞的毒性研究
- Author:
Jinming LI
;
Wei CHEN
;
Jiang LONG
;
Chen JIN
;
Weiyue LU
;
Quanxing NI
;
Deliang FU
;
Huimin HOU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Nanotechnology;
Albumin;
Gemcitabine;
Cytotoxicity
- From:
Chinese Journal of Pancreatology
2008;8(5):295-297
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate preparation of gemcitabine albumin nanoparticles, and its property of slow-release, the cytotoxic effect on pancreatic cancer cells (PANC1) in vitro, for improving the effect of regional intra-arterial infusion chemotherapy in pancreatic cancer with new medicament in the future. Methods The gemcitabine albumin nanoparticles were prepared with bovine serum albumin and gemcitabine with the desolvation-crosslink method, the concentration of gemcitabine was detected by high performance liquid chromatography (HPLC). The cytotoxic effect on pancreatic cancer cells in vitro were detected with MTT colorimetric assay. Results The mean diameter of gemcitabine albumin nanoparticles was (156.2±2.2) nm, and Zeta potential was (-20.4±1.41)mV, drug loading was 10.8%, drug release time in virto was 3 hours respectively. Gemcitabine albumin nanoparticles (0.01～50 μg/ml) had a 31%～44% inhibitory rate on PANC1 cell, which was similar to the inhibitory rate of same concentration of gemcitabine (26%～47%). Conclusions The new preparation of gemcitabine albumin nanoparticles had obvious drug slow-release effect, which may help improve the effect of regional intra-arterial infusion chemotherapy for pancreatic cancer.
