Testicular breeding sperm function and p63 expression in androgen receptor knockout mice
- VernacularTitle:雄激素受体敲除小鼠睾丸生精功能工能及63表达的改变
- Author:
Jianjun YU
;
Yuemin XU
;
Baojun GU
;
Hong XIE
;
Sanbao JIN
- Publication Type:Journal Article
- Keywords:
Androgen receptor;
Knockout;
Breeding sperm function;
p63
- From:
Chinese Journal of Urology
2008;29(z1):79-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the testicular breeding function and p63 expression in seminiferous tubules in androgen receptor(AR)knockout mice.Methods Eight AR knockout mice selected by Cre-lox and 8 Wistar male mice were studied.Testicular breeding function was determined by Makler score and p63 was detected by immunohistochemistry. Results Seminiferous tubules inner diameter deflated in AR knockout group than in Wistar group(60.0±1.5 μm vs 92.05±2.0μm,P＜0.01),peri-tube membrane thickened(4.0±0.7 μm vs 2.85=0.9 μm,P＜0.01),the number of cell layer decreased(2.0±0.3 vs 4.0±0.2,P＜0.01),generative eell maturation arrested(3.0±1.2 score vs 5.05±0.5 score,P＜0.01),Makler score was lower(8.0±0.3 vs 16.0±0.5,P＜0.01).p63 expression was lower in AR knockout group than in Wistar group and the expression was located at the stage of spermatogenous cell and spermatocyte.Conclusion Testicular breeding sperm function is damaged in AR knockout mice,lower expression of p63 mainly affects early differentiation in generative cells.
