Effect of peroxisome proliferator-activated receptor-γ ligand on acute necrotizing pancreatitis associated lung injury in rats
- VernacularTitle:PPAR-γ激动剂对急性坏死性胰腺炎大鼠肺损伤的作用
- Author:
Chen CHEN
;
Weixing WANG
;
Youming DING
;
Bin WANG
;
Sheng XU
;
Xiaoyan CHEN
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Peroxisome proliferator-activated receptors;
Tumor necrosis factor-α;
Lung injury;
Intercellular adhesion molecule-1
- From:
Chinese Journal of Pancreatology
2008;8(5):329-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Rosiglitazone on acute necrotizing pancreatitis (ANP) associated lung injury in rats and its potential mechanism. Methods Fifty-four male Wistar rats were randomly divided into sham operation group (SO group), ANP group and Rosiglitazone pretreatment group (Rosi group). ANP model was induced by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct. The rats of SO group, ANP group were injected with 10% dimethyl sulfoxide (DMSO) (0.2 ml/100 g) via femoral vein 30 minutes prior to the operation. In Resi group Rosiglitazone partes aequales (6 mg/kg), which was dissolved in 10% DMSO, was injected. Rats were killed at 3 h, 6 h and 12 h after induction. The serum amylase, lung myeloperoxidase (MPO), lung wet/dry (W/D) weight ratio, protein content of bronchoalveolar lavage fluids (BALF) were measured, and pancreas and lung pathologic changes were evaluated. RT-PCR was used for detecting the levels of TNF-α and ICAM-1 mRNA in lung tissue. Results In ANP group, the values a of serum amylase, MPO, W/D weight ratio, protein content of BALF, pancreas and lung pathologic score, lung TNF-α and ICAM-1mRNA expression increased corresponding to the duration of ANP course, and the corresponding values at 12 h were (5353.0±728.2) U/L, (1.12±0.14) U/g, 3.00±0.14, 0. 438±0.056, 11.17±0.93 and 8.17±0.75, respectively, which were significantly higher than those in the SO group (P<0. 05 or P<0.01). The corresponding values in the Rosi group were (2847.4±841) U/L, (0.84±0.06) U/g, 2.13±0.36, 0.283±0.078 g/L, 7.75±0.27, 4.33±0.82, 0.26±0.04 and 0.84 ±0.02, respectively, which were significantly higher than those in the SO group (P <0. 05 or P <0.01). Conclusions Rosiglitazone had protective effect on lung injuries in ANP rats through down-regnlating the expression of TNF-α and ICAM-1 mRNA.
