Effects of resveratrol on the expressions of E-selectin and monocyte chemoattractant protein-1 of endothelial cells
- VernacularTitle:白藜芦醇对内皮细胞E-选择素和巨噬细胞趋化蛋白-1表达的影响
- Author:
Pengli ZHU
;
Dean JIA
;
Yanghui SHEN
;
Jingming RUAN
;
Huizhen YU
;
Hui CHEN
- Publication Type:Journal Article
- Keywords:
Endothelial cells;
Atherosclerosis;
E-selectin;
Chemotactic factors,macrophage
- From:
Chinese Journal of Geriatrics
2008;27(11):811-814
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of resveratrol on the expressions of E-selectin and monocyte chemoattractant protein-1 (MCP-1) in activated endothelial cells.Methods After being pretreated with resveratrol followed by tumor necrosis factor-α (TNF-α) stimulation, human umbilical vein endothelial cells (HUVEC) were randomly divided into three groups: TNF group,resveratrol+TNF-α group and control group. The expression of E-selectin molecule on the surface of HUVEC was detected by flow cytometric analysis and the mRNA expressions of E-selectin and MCP -1 were determined by semiquantitative RT-PCR. ResultsTNF-α induced the expression of E-selectin and MCP-I of HUVEC.Resveratrol (10 μmol/L) inhibited E-selectin expression.The positive cells of E-selectin in TNF group, resveratrol + TNF-α group and control group were(47.84±3.2)%, (15.3±1.7)% and (3.74±1.6)%, respectively, and the differences among the three groups were statistically significant (P<0.05). Conclusions Resveratrol may contribute to the anti-atherosclerotic effect by inhibiting the expression of E-seleetin and MCP-1 of HUVEC.
