Application of flow-through hybrization and gene chipon human papiliomavious subtype detection in Chongqing
- VernacularTitle:运用导流杂交基因芯片技术在重庆地区人乳头瘤病毒感染分型检测的研究
- Author:
Fang CUI
;
Yin JIA
;
Jin WU
;
Liping ZHANG
- Publication Type:Journal Article
- Keywords:
Papillomavirus infections;
Nucleic acid hybridization;
DNA probes,HPV;
Polymerase chain reaction
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(9):1449-1451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of flow-through rapid hybridization technique and gene chip (HybriMax) on human papiUomavions (HPV) subtype in Chongqing.Methods Cervical tissue samples were taken under the colpnscope form 473 females who had cervical lesion for pathological analysis.The predictive value of HybriMax in cervical abnormality was compared with pathological results,which were used as golden standard.Resuits 13 different subtypes were found and total HPV positive rate was 63.0% (284/473) Among the 17 different subtypes ,the higher positive rote HPV subtypes were HPV16 (23.7%,112/473),HPV58 (12.7% ,60/473),HPV53(7.4% ,35/473).The HPV infection rates were higher with the worse d cervical lesion(X2=77.06,P＜0.01).Conclusions The most frequent subtypes of HPV infection in Chongqing cervical lesion were HPV 16,58.HybriMax was an effective method to detect HPV subtype in clinical.
